Single-cell transcriptomics reveals unique features of human pancreatic islet cell subtypes

As the detailed reports are pasted below and have also been forwarded to you prior to this email, I will only repeat the main point(s) here. As we have discussed before, two referees (1 and 2) raised concerns about the purity and definition of the different cell types based on established marker gene expression and referee 1 still feels that your explanation does not sufficiently address this point (his/her additional comments are also pasted below for your information). S/he suggests using additional, classical marker genes and additional controls to unambiguously rule out crosscontamination and/or mis-classifications. Referee 3 also agrees that double marker analysis would be more informative in this regard.